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Research

Leica SP5 Confocal


An upright laser scanning confocal microscope equipped for high temporal and spatial resolution imaging of fixed cells or tissue sections, as well as live tissue and in vivo intravital techniques.

 

Specification:

For a full system specification, visit the CMR Advanced Bio-imaging Facility website. In brief, the system is configured with seven laser lines (458, 476, 488, 496, 514, 561 and 633 m), four objective lens options (10x/0.3 W Apo, 20x/1.0 W Apo, 63x/0.9 W Apo, 63x/1.4 oil Pl Apo), and three PMT detectors for fast multi-channel capture. 

 

Applications:

With this confocal laser scanning technology, high-quality optical Z-sections can be acquired to capture high-resolution multi-channel 3/4D images of fixed or live samples. The large stage area, water dipping lenses and ultra-fast resonant scanner, make this system also suitable for the challenges of live tissue and intravital imaging experiments. The long working distance collection of objective lenses (up to 3.6 mm) are additionally well suited to deep imaging within cleared tissues and its large stage area can accommodate large or unusal sample types that don't have to conform to the standard slide or imaging chamber mount. 

Costs

Price per hour : £25

Price O/N : n/a

Contact details

Facility : CMR Advanced Bio-imaging Facility

Campus : Charterhouse Square

Address : Centre for Microvascular Research, William Harvey Research Institute, Charterhouse Square, EC1M 6BQ

Manager : Dr Rebecca Saleeb

Contact : 020 7882 3910

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