Olympus iX81 Epifluorescence Microscope

This Olympus iX81 is an inverted, fully motorised and environment-controlled microscope for brightfield, phase contrast and epifluorescence imaging applications, suited to live and fixed samples on slides, 35 mm dishes or multi-well plates. 

 

Specification:

For a full system specification, visit the CMR Advanced Bio-imaging Facility equipment pages. In brief, the system is configured with DAPI/GFP/RFP LEDs and filtersets, four dry phase objective lens options (4x, 10x, 20x, 40x), a Hamamatsu Orca-ER camera and it runs off the latest CellSens software.

 

Applications:

As a camera-based system, widefield microscopy provides high-speed capture that is ideally suited to thin samples (cell monolayers or 10 um tissue sections). Common applications include label-free multi-well cell migration assays by phase contrast, tile and stitch scans to map fluorescence over large tissue sections and low-cost sample screening ahead of confocal work.